Okuqukethwe
Ukugqugquzela i-Follicular
Ngaphambili, umama ozozala kumele athole ukwelashwa ngama-hormone ilawulwa ngomjovo. Umgomo walokhu: ukuthola ukuthuthukiswa kwama-follicle amaningi okuvumela ukuqoqwa kwama-oocyte amaningana. Uma kukhona, maningi amathuba okukhulelwa. Ukukhuthazwa kuqashwe ngokuqinile (ukuqapha) ngu ama-ultrasounds futhi ukuhlolwa kwe-hormonal. Lapho ama-follicles ekhula, i-ovulation ibangelwa umjovo wamahomoni ngomsebenzi we-LH: i-hCG.
Ukubhoboza ama-oocyte
Phakathi kwamahora angu-36 no-40 ngemva kokuqalwa kwe-ovulation, ama-ovarian follicles abhobozwa nge-transvaginally. Ngokunemba kakhudlwana, uketshezi oluqukethwe ku-follicle ngayinye equkethe ama-oocyte avuthiwe ashiswa kusetshenziswa inaliti. Ukubhoboza kwenziwa ngaphansi kokulawulwa kwe-ultrasound futhi kwenzeka ngaphansi kwe-anesthesia yendawo noma, kaningi, ngaphansi kwe-anesthesia jikelele.
Ukulungiswa kwama-oocyte
Khona-ke uketshezi lwe-follicular luyahlolwa elabhorethri ukuze kutholakale ama-oocyte futhi luwahlukanise. Kufanele wazi ukuthi wonke ama-follicle awaqukethe i-oocyte nalokho akuwona wonke ama-oocyte avundiswayo.
Ukulungisa isidoda
Ukuqoqwa kwesidoda nokulungiswa kwaso (kuyawashwa) kuvame ukwenziwa ngosuku lwe-IVF elabhorethri. THEkuzokhethwa isidoda esinyakazayo kakhulu. Ngenxa yezizathu ezihlukahlukene, kungenzeka ukuthi isidoda siqoqwe kahle ngaphambili; ngakho-ke zizoba yiqhwa. Endabeni yokungazali okukhulu kwabesilisa, kungase kudingeke ukubhoboza ngokuhlanganyela ama-oocyte kanye nesidoda (ama-epididymal noma ama-testicular punctures).
Ukufakwa amadlozi
Iku-a isitsha sokudla esiqukethe uketshezi olunomsoco ukuthi ukuxhumana phakathi kwe-spermatozoa nama-oocyte kwenzeka. Lokhu kufakwa ngaphakathi kwe-incubator ku-37 ° C. Lesi sakamuva kufanele sibe buthaka igobolondo le-oocyte ukuze omunye wabo akwazi ukulivundisa.
Ukuvundisa nokukhula kombungu
Ngosuku olulandelayo, singabona ukuthi akhona yini ama-oocyte avundisiwe. Ukuze wazi kahle inani lemibungu etholiwe, kuyadingeka ukulinda amahora angama-24 ngaphezulu. Uma ukuvundiswa sekwenzekile, imibungu enamaseli angu-2, 4, 6 noma 8 ingabonwa (inani lamaseli lincike ngosuku eyabonwa ngalo). Imibungu evamile kakhulu idluliswa ezinsukwini ezingu-2-3 ngemva kokubhoboza noma iqhwa.
Zingenziwa futhi ukuthi ziguquke isikhathi eside endaweni yesiko ende ukuze zifinyelele esigabeni “se-blastocyst”, isigaba sokugcina sokukhula ngaphambi kokuchanyuselwa.
Ukudluliswa kombungu
Lesi senzo esingenabuhlungu futhi esisheshayo senziwa elabhorethri ye-IVF. Ukusebenzisa i-catheter encane, i-e noma imibungu ifakwa ngaphakathi kwesibeletho. Imvamisa kudluliswa umbungu owodwa noma emibili bese kuthi eminye iqandiswe uma izinga layo livuma. Ngemuva kwalesi senzo, isigaba se-luteal sisekelwa ukunikezwa kwansuku zonke kwe-progesterone.
Ukuqapha ukukhulelwa
Ukukhulelwa kuphawulwa ngu-a umthamo we-hormonal ohlelekile cishe ngosuku lweshumi nantathu ngemva kokudluliswa kombungu (ku-IVF kungase kube nokopha okungenangqondo okungafihla ukuqala kokukhulelwa).
Kuthiwani nge-IVF nge-ICSI?
Ngesikhathi se-IVF nge-ICSI (umjovo wesidoda we-intracytoplasmic), ikakhulukazi ohloselwe ukungazali kwabesilisa, indlela ihluke kancane. Kukhethwa isidoda esisodwa kuphela. Ibe isijovwa ngaphakathi kwe-oocyte nasendaweni ethile. Ngemuva kwamahora angu-19-20, ukutholakala kwama-nuclei amabili kuyahlolwa.